Big Idea: Biotechnology
a. Exhibit the above traits in their own scientific activities.
b. Recognize that different explanations often can be given for the same evidence.
c. Explain that further understanding of scientific problems relies on the design and execution of new experiments which may reinforce or weaken opposing explanations.
a. Follow correct procedures for use of scientific apparatus.
b. Demonstrate appropriate technique in all laboratory situations.
c. Follow correct protocol for identifying and reporting safety problems and violations.
c. Collect, organize and record appropriate data.
a. Develop and use systematic procedures for recording and organizing information.
b. Use technology to produce tables and graphs.
c. Use technology to develop, test, and revise experimental or mathematical models.
a. Trace the source on any large disparity between estimated and calculated answers to problems.
b. Consider possible effects of measurement errors on calculations.
a. Write clear, coherent laboratory reports related to scientific investigations.
b. Write clear, coherent accounts of current scientific issues, including possible alternative interpretations of the data.
c. Use data as evidence to support scientific arguments and claims in written or oral presentations.
d. Participate in group discussions of scientific investigation and current scientific issues.
b. Explain the role of DNA in storing and transmitting cellular information.
f. Examine the use of DNA technology in forensics, medicine, and agriculture.
This laboratory exercise consists of six parts.
Students will isolate bacteria
Students will extract DNA
Students will use PCR to amplify the bacterial DNA.
Students will use gel electrophoresis to detect DNA in their samples.
Students will learn about Bioinformatics
Students will BLAST search their sequences at GenBank
What are the practical applications of DNA technology?
See Habits of Mind SCSh2
Through this laboratory activity, observations of the students performing the different techniques will be used as formative assessment. The summative assessment or overall final assessment is the laboratory report the students will turn in at the end of the activity.